Alakpa, E. V., Jayawarna, V., Burgess, K. E.V., West, C. C., Péault, B., Ulijn, R. V. and Dalby, M. J. (2017) Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels. Scientific Reports, 7, 6895. (doi: 10.1038/s41598-017-07255-z) (PMID:28761049) (PMCID:PMC5537289)
|
Text
143128.pdf - Published Version Available under License Creative Commons Attribution. 2MB |
Abstract
Differentiation of stem cells to chondrocytes in vitro usually results in a heterogeneous phenotype. This is evident in the often detected over expression of type X collagen which, in hyaline cartilage structure is not characteristic of the mid-zone but of the deep-zone ossifying tissue. Methods to better match cartilage developed in vitro to characteristic in vivo features are therefore highly desirable in regenerative medicine. This study compares phenotype characteristics between pericytes, obtained from human adipose tissue, differentiated using diphenylalanine/serine (F2/S) peptide hydrogels with the more widely used chemical induced method for chondrogenesis. Significantly higher levels of type II collagen were noted when pericytes undergo chondrogenesis in the hydrogel in the absence of induction media. There is also a balanced expression of collagen relative to aggrecan production, a feature which was biased toward collagen production when cells were cultured with induction media. Lastly, metabolic profiles of each system show considerable overlap between both differentiation methods but subtle differences which potentially give rise to their resultant phenotype can be ascertained. The study highlights how material and chemical alterations in the cellular microenvironment have wide ranging effects on resultant tissue type.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Dalby, Professor Matthew and Burgess, Dr Karl and Jayawarna, Dr Vineetha |
| Authors: | Alakpa, E. V., Jayawarna, V., Burgess, K. E.V., West, C. C., Péault, B., Ulijn, R. V., and Dalby, M. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Scientific Reports |
| Publisher: | Nature Publishing Group |
| ISSN: | 2045-2322 |
| ISSN (Online): | 2045-2322 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Scientific Reports 7: 6895 |
| Publisher Policy: | Reproduced under a Creative Commons license |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
Funder and Project Information