Rodie, M.E., Mudaliar, M.A.V. , Herzyk, P. , McMillan, M., Boroujerdi, M., Chudleigh, S., Tobias, E.S. and Ahmed, S.F. (2017) Androgen-responsive non-coding small RNAs extend the potential of HCG stimulation to act as a bioassay of androgen sufficiency. European Journal of Endocrinology, 177(4), pp. 339-346. (doi: 10.1530/EJE-17-0404) (PMID:28733293)
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Abstract
Background: It is unclear whether a short-term change in circulating androgens is associated with changes in the transcriptome of the peripheral blood mononuclear cells (PBMC). Aims & Methods: To explore the effect of hCG-stimulation on the PBMC-transcriptome, 12 boys with a median age (range) of 0.7yrs (0.3, 11.2) who received intramuscular hCG 1500u on 3 consecutive days as part of their investigations underwent transcriptomic array analysis on RNA extracted from peripheral blood mononuclear cells before and after hCG stimulation. Results: Median pre and post hCG testosterone for the overall group was 0.7nmol/l (<0.5,6) and 7.9nmol/l (<0.5, 31.5), respectively. Of the 12 boys, 3 (25%) did not respond to hCG stimulation with a pre and post median serum testosterone of <0.5nmol/l and <0.5nmol/l, respectively. When corrected for gene expression changes in the non-responders to exclude hCG effects, all 9 of the hCG responders consistently demonstrated a 20% or greater increase in the expression of piR-37153 and piR-39248, non-coding PIWI-interacting RNAs (piRNAs). In addition, of the 9 responders, 8, 6 and 4 demonstrated a 30%, 40% and 50% rise, respectively in a total of 2 further piRNAs. In addition, 3 of the responders showed a 50% or greater rise in the expression of another small RNA, SNORD5. On comparing fold change in serum testosterone with fold change in the above transcripts, a positive correlation was detected for SNORD5 (p=0.01). Conclusions: The identification of a dynamic and androgen-responsive PBMC-transcriptome extends the potential value of the hCG test for assessment of androgen sufficiency.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Ahmed, Professor Syed Faisal and Boroujerdi, Dr Massoud and Rodie, Dr Martina and Tobias, Professor Edward and McMillan, Mr Martin and Mudaliar, Dr Manikhandan and Herzyk, Dr Pawel |
Authors: | Rodie, M.E., Mudaliar, M.A.V., Herzyk, P., McMillan, M., Boroujerdi, M., Chudleigh, S., Tobias, E.S., and Ahmed, S.F. |
College/School: | College of Medical Veterinary and Life Sciences > School of Health & Wellbeing > MRC/CSO SPHSU College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | European Journal of Endocrinology |
Publisher: | BioScientifica |
ISSN: | 0804-4643 |
ISSN (Online): | 1479-683X |
Published Online: | 21 July 2017 |
Copyright Holders: | Copyright © 2017 The Authors |
First Published: | First published in European Journal of Endocrinology 177(4): 339-346 |
Publisher Policy: | Reproduced under a Creative Commons License |
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