Cell migration on material-driven fibronectin microenvironments

Grigoriou, E. , Cantini, M. , Dalby, M.J. , Petersen, A. and Salmeron-Sanchez, M. (2017) Cell migration on material-driven fibronectin microenvironments. Biomaterials Science, 5(7), 1326. (doi: 10.1039/C7BM00333A) (PMID:28612879) (PMCID:PMC5858633)

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Cell migration is a fundamental process involved in a wide range of biological phenomena. However, how the underlying mechanisms that control migration are orchestrated is not fully understood. In this work, we explore the migratory characteristics of human fibroblasts using different organisations of fibronectin (FN) triggered by two chemically similar surfaces, poly(ethyl acrylate) (PEA) and poly(methyl acrylate) (PMA); cell migration is mediated via an intermediate layer of fibronectin (FN). FN is organised into nanonetworks upon simple adsorption on PEA whereas a globular conformation is observed on PMA. We studied cell speed over the course of 24 h and the morphology of focal adhesions in terms of area and length. Additionally, we analysed the amount of cell-secreted FN as well as FN remodelling. Velocity of human fibroblasts was found to exhibit a biphasic behaviour on PEA, whereas it remained fairly constant on PMA. FA analysis revealed more mature focal adhesions on PEA over time contrary to smaller FAs found on PMA. Finally, human fibroblasts seemed to remodel adsorbed FN more on PMA than on PEA. Overall, these results indicate that the cell–protein–material interface affects cell migratory behaviour. Analysis of FAs together with FN secretion and remodelling were associated with differences in cell velocity providing insights into the factors that can modulate cell motility.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Dalby, Professor Matthew and Grigoriou, Eleni and Cantini, Dr Marco
Authors: Grigoriou, E., Cantini, M., Dalby, M.J., Petersen, A., and Salmeron-Sanchez, M.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Biomaterials Science
Publisher:Royal Society of Chemistry
Published Online:06 June 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Biomaterials Science 5(7):1326
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.1039/C7BM00333A

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
626901HEALINSYNERGY - Material-driven fibronectin fibrillogenesis to engineer synergistic growth factor microenvironmentsManuel Salmeron-SanchezEuropean Research Council (ERC)306990ENG - BIOMEDICAL ENGINEERING
653731Synergistic microenvironments for non-union bone defectsMatthew DalbyMedical Research Council (MRC)MR/L0022710/1RI MOLECULAR CELL & SYSTEMS BIOLOGY