Comparative study of osteogenic activity of multilayers made of synthetic and biogenic polyelectrolytes

Guduru, D., Niepel, M. S., Gonzalez-Garcia, C., Salmeron-Sanchez, M. and Groth, T. (2017) Comparative study of osteogenic activity of multilayers made of synthetic and biogenic polyelectrolytes. Macromolecular Bioscience, 17(8), 1700078. (doi: 10.1002/mabi.201700078) (PMID:28547877)

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Polyelectrolyte multilayer (PEM) coatings on biomaterials are applied to tailor adhesion, growth, and function of cells on biomedical implants. Here, biogenic and synthetic polyelectrolytes (PEL) are used for layer-by-layer assembly to study the osteogenic activity of PEM with human osteosarcoma MG-63 cells in a comparative manner. Formation of PEM is achieved with biogenic PEL fibrinogen (FBG) and poly-l-lysine (PLL) as well as biotinylated chondroitin sulfate (BCS) and avidin (AVI), while poly(allylamine hydrochloride) (PAH) and polystyrene sulfonate (PSS) represent a fully synthetic PEM used as a reference system here. Surface plasmon resonance measurements show highest layer mass for FBG/PLL and similar for PSS/PAH and BCS/AVI systems, while water contact angle and zeta potential measurements indicate larger differences for PSS/PAH and FBG/PLL but not for BCS/AVI multilayers. All PEM systems support cell adhesion and growth and promote osteogenic differentiation as well. However, FBG/PLL layers are superior regarding MG-63 cell adhesion during short-term culture, while the BCS/AVI system increases alkaline phosphatase activity in long-term culture. Particularly, a multilayer system based on affinity interaction like BCS/AVI may be useful for controlled presentation of biotinylated growth factors to promote growth and differentiation of cells for biomedical applications.

Item Type:Articles
Keywords:Biotechnology, materials chemistry, bioengineering, polymers and plastics, biomaterials.
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Gonzalez Garcia, Dr Cristina
Authors: Guduru, D., Niepel, M. S., Gonzalez-Garcia, C., Salmeron-Sanchez, M., and Groth, T.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Macromolecular Bioscience
ISSN (Online):1616-5195
Published Online:26 May 2017
Copyright Holders:Copyright © 2017 Wiley-VCH Verlag GmbH & Co.
First Published:First published in Macromolecular Bioscience 17(8): 1700078
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
626901HEALINSYNERGY - Material-driven fibronectin fibrillogenesis to engineer synergistic growth factor microenvironmentsManuel Salmeron-SanchezEuropean Research Council (ERC)306990ENG - BIOMEDICAL ENGINEERING