Hock, A. K. et al. (2017) Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo. Scientific Reports, 7, 1837. (doi: 10.1038/s41598-017-01741-0) (PMID:28500323) (PMCID:PMC5431786)
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Abstract
While the use of bioluminescent proteins for molecular imaging is a powerful technology to further our understanding of complex processes, fluorescent labeling with visible light fluorescent proteins such as GFP and RFP suffers from poor tissue penetration and high background autofluorescence. To overcome these limitations, we generated an inducible knock-in mouse model of iRFP713. This model was used to assess Cre activity in a Rosa Cre-ER background and quantify Cre activity upon different tamoxifen treatments in several organs. We also show that iRFP can be readily detected in 3D organoid cultures, FACS analysis and in vivo tumour models. Taken together we demonstrate that iRFP713 is a progressive step in in vivo imaging and analysis that widens the optical imaging window to the near-infrared spectrum, thereby allowing deeper tissue penetration, quicker image acquisition without the need to inject substrates and a better signal to background ratio in genetically engineered mouse models (GEMMs).
Item Type: | Articles |
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Additional Information: | Funding was provided by CRUK core grant A18274, the Cancer Research UK Glasgow Centre (C596/A18076) and the BSU facilities at the Cancer Research UK Beatson Institute (C596/A17196). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | McGhee, Dr Ewan and Blyth, Professor Karen and Vousden, Karen and Murphy, Professor Daniel and Cheung, Mr Eric and Paulus-Hock, Mrs Viola and Strathdee, Mr Douglas and Hock, Dr Andreas and Scopelliti, Mr Alessandro |
Authors: | Hock, A. K., Cheung, E. C., Humpton, T. J., Monteverde, T., Paulus-Hock, V., Lee, P., McGhee, E., Scopelliti, A., Murphy, D. J., Strathdee, D., Blyth, K., and Vousden, K. H. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Journal Name: | Scientific Reports |
Publisher: | Nature Research |
ISSN: | 2045-2322 |
ISSN (Online): | 2045-2322 |
Copyright Holders: | Copyright © 2017 The Authors |
First Published: | First published in Scientific Reports 7: 1837 |
Publisher Policy: | Reproduced under a Creative Commons License |
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