Baumgartner, H. K., Rudolph, M. C., Ramanathan, P., Burns, V., Webb, P., Bitler, B. G., Stein, T. , Kobayashi, K. and Neville, M. C. (2017) Developmental expression of claudins in the mammary gland. Journal of Mammary Gland Biology and Neoplasia, 22(2), pp. 141-157. (doi: 10.1007/s10911-017-9379-6) (PMID:28455726)
|
Text
140790.pdf - Published Version Available under License Creative Commons Attribution. 3MB |
Abstract
Claudins are a large family of membrane proteins whose classic function is to regulate the permeability of tight junctions in epithelia. They are tetraspanins, with four alpha-helices crossing the membrane, two extracellular loops, a short cytoplasmic N-terminus and a longer and more variable C-terminus. The extracellular ends of the helices are known to undergo side-to-side (cis) interactions that allow the formation of claudin polymers in the plane of the membrane. The extracellular loops also engage in head-to-head (trans) interactions thought to mediate the formation of tight junctions. However, claudins are also present in intracellular structures, thought to be vesicles, with less well-characterized functions. Here, we briefly review our current understanding of claudin structure and function followed by an examination of changes in claudin mRNA and protein expression and localization through mammary gland development. Claudins-1, 3, 4, 7, and 8 are the five most prominent members of the claudin family in the mouse mammary gland, with varied abundance and intracellular localization during the different stages of post-pubertal development. Claudin-1 is clearly localized to tight junctions in mammary ducts in non-pregnant non-lactating animals. Cytoplasmic puncta that stain for claudin-7 are present throughout development. During pregnancy claudin-3 is localized both to the tight junction and basolaterally while claudin-4 is found only in sparse puncta. In the lactating mouse both claudin-3 and claudin-8 are localized at the tight junction where they may be important in forming the paracellular barrier. At involution and under challenge by lipopolysaccharide claudins -1, -3, and -4 are significantly upregulated. Claudin-3 is still colocalized with tight junction molecules but is also distributed through the cytoplasm as is claudin-4. These largely descriptive data provide the essential framework for future mechanistic studies of the function and regulation of mammary epithelial cell claudins.
| Item Type: | Articles |
|---|---|
| Keywords: | Claudin-1, claudin-3, claudin-4, claudin-7, claudin-8, extra-junctional claudin, infection, involution, mammary gland. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Stein, Dr Torsten |
| Authors: | Baumgartner, H. K., Rudolph, M. C., Ramanathan, P., Burns, V., Webb, P., Bitler, B. G., Stein, T., Kobayashi, K., and Neville, M. C. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
| Journal Name: | Journal of Mammary Gland Biology and Neoplasia |
| Publisher: | Springer |
| ISSN: | 1083-3021 |
| ISSN (Online): | 1573-7039 |
| Published Online: | 28 April 2017 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Journal of Mammary Gland Biology and Neoplasia 22(2):141-157 |
| Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record
Deposit and Record Details
Deposit and Record Details