Multispectral oximetry of murine tendon microvasculature with inflammation

van der Putten, M. , Brewer, J. M. and Harvey, A. R. (2017) Multispectral oximetry of murine tendon microvasculature with inflammation. Biomedical Optics Express, 8(6), pp. 2896-2905. (doi: 10.1364/BOE.8.002896)

Abstract

We report a novel multispectral imaging technique for localised measurement of vascular oxygen saturation (SO2) in vivo. Annular back-illumination is generated using a Schwarzchild-design reflective objective. Analysis of multispectral data is performed using a calibration-free oximetry algorithm. This technique is applied to oximetry in mice to measure SO2 in microvasculature supplying inflamed tendon tissue in the hind leg. Average SO2 for controls was 94.8 ± 7.0 % (N = 6), and 84.0 ± 13.5 % for mice with inflamed tendon tissue (N = 6). We believe this to be the first localised measurement of hypoxia in tendon microvasculature due to inflammation. Quantification of localised SO2 is important for the study of inflammatory diseases such as rheumatoid arthritis, where hypoxia is thought to play a role in pathogenesis.

• Item Type: Articles
• Additional Information: Funded by a Lord Kelvin Adam Smith Scholarship (LKAS), University of Glasgow.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Brewer, Professor James and Harvey, Professor Andy and Van Der Putten, Miss Marieke
• Authors: van der Putten, M., Brewer, J. M., and Harvey, A. R.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Science and Engineering > School of Physics and Astronomy
• Journal Name: Biomedical Optics Express
• Publisher: Optical Society of America
• ISSN: 2156-7085
• ISSN (Online): 2156-7085
• Published Online: 08 May 2017
• Copyright Holders: Copyright © 2017 Optical Society of America
• First Published: First published in Biomedical Optics Express 8(6):2896-2905
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher