Ho, K. L., Kueh, C. L., Beh, P. L., Tan, W. S. and Bhella, D. (2017) Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution. Scientific Reports, 7, 2083. (doi: 10.1038/s41598-017-02292-0) (PMID:28522842) (PMCID:PMC5437026)
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Abstract
White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. There are no interventions available to treat or prevent MrNV disease however. Here we show the structure of MrNV virus-like particles (VLPs) produced by recombinant expression of the capsid protein, using cryogenic electron microscopy. Our data show that MrNV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T = 3 icosahedral virus particles. MrNV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell. Our structural analysis supports the assertion that MrNV may belong to a new genus of the Nodaviridae. Moreover, our study provides the first structural view of an important pathogen affecting aquaculture industries across the world.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ho, Dr Kok Lian and Bhella, Professor David |
| Authors: | Ho, K. L., Kueh, C. L., Beh, P. L., Tan, W. S., and Bhella, D. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Scientific Reports |
| Publisher: | Nature Publishing Group |
| ISSN: | 2045-2322 |
| ISSN (Online): | 2045-2322 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Scientific Reports 7:2083 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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