Martin, K. C., Yuan, X. , Stimac, G., Bannerman, K., Anderson, J., Roy, C., Glykofrydis, F., Yin, H. and Davies, J. A. (2017) Symmetry-breaking in branching epithelia: cells on micro-patterns under flow challenge the hypothesis of positive feedback by a secreted autocrine inhibitor of motility. Journal of Anatomy, 230(6), pp. 766-774. (doi: 10.1111/joa.12599) (PMID:28369863)
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Abstract
Branching morphogenesis of epithelia involves division of cells into leader (tip) and follower (stalk) cells. Published work on cell lines in culture has suggested that symmetry-breaking takes place via a secreted autocrine inhibitor of motility, the inhibitor accumulating more in concave regions of the culture boundary, slowing advance of cells there, and less in convex areas, allowing advance and a further exaggeration of the concave/convex difference. Here we test this hypothesis using a two-dimensional culture system that includes strong flow conditions to remove accumulating diffusible secretions. We find that, while motility does indeed follow boundary curvature in this system, flow makes no difference: this challenges the hypothesis of control by a diffusible secreted autocrine inhibitor.
| Item Type: | Articles |
|---|---|
| Keywords: | Autocrine secretion, branching morphogenesis, cell motility, positive feedback, self-organisation, shape. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Yin, Professor Huabing and Yuan, Dr Xiaofei |
| Authors: | Martin, K. C., Yuan, X., Stimac, G., Bannerman, K., Anderson, J., Roy, C., Glykofrydis, F., Yin, H., and Davies, J. A. |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Journal of Anatomy |
| Publisher: | Wiley |
| ISSN: | 0021-8782 |
| ISSN (Online): | 1469-7580 |
| Published Online: | 29 March 2017 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Journal of Anatomy 230(6):766-774 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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