T cell response in the lung following influenza virus infection

Benson, R. A. , Lawton, J. C. and MacLeod, M. K.L. (2017) T cell response in the lung following influenza virus infection. In: Rainger, G. E. and Mcgettrick, H. M. (eds.) T-Cell Trafficking: Methods and Protocols. Series: Methods in molecular biology (1591). Springer: New York, pp. 235-248. ISBN 9781493969296 (doi: 10.1007/978-1-4939-6931-9_17)

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Methods that enable the identification of virus-specific CD4 and CD8 T cells are key to our understanding of how the adaptive immune response controls viral infection. Here we describe two distinct methods to evaluate the T cell response to influenza A virus (IAV). The number and phenotype of T cells that respond to natural IAV epitopes can be assessed by flow cytometry using MHC class I and class II tetramers. Using this system, IAV-specific T cells can be tracked in various organs within the same animal, or, in different cohorts, the response can be evaluated at several time points following infection. While providing clear quantitative data, flow cytometry cannot provide any information about T cell location within the lung or interactions between responding T cells and other cell types. Here we also describe a method to examine activated CD4 T cells in the lungs of living animals using multiphoton intravital microscopy, thus providing real-time analysis of T cell behavior during an infection.

Item Type:Book Sections
Keywords:Infection, Influenza A virus, intravital imaging, lung, MHC tetramers, memory, T cells.
Glasgow Author(s) Enlighten ID:Macleod, Dr Megan and Benson, Dr Robert and Lawton, Dr Jennifer
Authors: Benson, R. A., Lawton, J. C., and MacLeod, M. K.L.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity

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