Use of hyaluronidase in the comparison between manual and automated hematology analysis with the ADVIA 120 to improve analysis of feline body cavity effusions

Lee, S. M., Williamson, K., Weir, W. , Hulme-Moir, L. and Haining, H. (2017) Use of hyaluronidase in the comparison between manual and automated hematology analysis with the ADVIA 120 to improve analysis of feline body cavity effusions. Journal of Veterinary Diagnostic Investigation, 29(2), pp. 212-216. (doi: 10.1177/1040638716685133) (PMID:28064834)

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Classification of body cavity effusions is an important step in the investigation and diagnosis of disease in cats. Feline inflammatory effusions are often highly proteinaceous and viscous, which can cause clumping of white cells and subsequently inaccurate nucleated cell counts (NCCs) using automated and manual methods. Microscopic assessment of cellularity can also be difficult given the variable thickness of smears and cell clumping, which skews white cell distribution. The ADVIA 120 uses 2 white cell–counting channels, the basophil/lobularity (WBC/baso) and differential/peroxidase channels, which can provide quite different results in highly viscous feline samples and often disagree with smear assessment of cellularity. We investigated the effects of pre-incubation of feline effusion samples with hyaluronidase and its effects on NCCs and cellularity assessment. NCCs were obtained by automated analysis using the ADVIA 120 and by manual counting methods. Agreement was assessed using a Bland–Altman chart. Pretreatment of samples with hyaluronidase resulted in good agreement between the ADVIA basophil channel and manual counting methods in all samples in the study. However, improvements in NCCs after hyaluronidase treatment were significantly greater in clumped samples, and cell distribution of these samples on direct smears was also improved. Therefore, when nucleated cell clumping is observed on a direct smear, pretreatment of the sample with hyaluronidase prior to analysis on an automated analyzer is advised, with the WBC/baso channel displaying the most accurate NCC.

Item Type:Articles
Additional Information:This study was funded by the University of Glasgow Veterinary School student summer vacation scholarship program.
Glasgow Author(s) Enlighten ID:Haining, Dr Hayley and Williamson, Mr Kenny and Weir, Professor Willie
Authors: Lee, S. M., Williamson, K., Weir, W., Hulme-Moir, L., and Haining, H.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Journal of Veterinary Diagnostic Investigation
Publisher:SAGE Publications
ISSN (Online):1943-4936
Published Online:08 January 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Journal of Veterinary Diagnostic Investigation 29(2): 212-216
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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