NEDD8 overexpression results in neddylation of ubiquitin substrates by the ubiquitin pathway

Hjerpe, R., Thomas, Y. and Kurz, T. (2012) NEDD8 overexpression results in neddylation of ubiquitin substrates by the ubiquitin pathway. Journal of Molecular Biology, 421(1), pp. 27-29. (doi: 10.1016/j.jmb.2012.05.013) (PMID:22608973)

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Ubiquitin and ubiquitin-like proteins use unique E1, E2, and E3 enzymes for conjugation to their substrates. We and others have recently reported that increases in the relative concentration of the ubiquitin-like protein NEDD8 over ubiquitin lead to activation of NEDD8 by the ubiquitin E1 enzyme. We now show that this results in erroneous conjugation of NEDD8 to ubiquitin substrates, such as p53, Caspase 7, and Hif1α, demonstrating that overexpression of NEDD8 is not appropriate for identification of substrates of the NEDD8 pathway.

Item Type:Articles
Additional Information:This work was supported by an ERC Young Investigator Grant (to T.K.), by a European Regional Development Fund Grant for an Innovation Pipeline for Translational Science [grant number LUPS/ERDF/ 2008/2/1/0429] and by a grant from the Scottish government to the Scottish Institute for Cell Signalling.
Glasgow Author(s) Enlighten ID:Kurz, Dr Thimo
Authors: Hjerpe, R., Thomas, Y., and Kurz, T.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Journal of Molecular Biology
ISSN (Online):1089-8638
Published Online:15 May 2012
Copyright Holders:Copyright © 2012 Elsevier Ltd.
First Published:First published in Journal of Molecular Biology 421(1): 27-29
Publisher Policy:Reproduced under a Creative Commons License

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