Requirement of Ras-GTP-Raf complexes for activation of Raf-1 by protein kinase C

Marais, R., Light, Y., Mason, C., Paterson, H., Olson, M. F. and Marshall, C. J. (1998) Requirement of Ras-GTP-Raf complexes for activation of Raf-1 by protein kinase C. Science, 280(5360), pp. 109-112. (doi: 10.1126/science.280.5360.109) (PMID:9525855)

Abstract

Receptor tyrosine kinase–mediated activation of the Raf-1 protein kinase is coupled to the small guanosine triphosphate (GTP)–binding protein Ras. By contrast, protein kinase C (PKC)–mediated activation of Raf-1 is thought to be Ras independent. Nevertheless, stimulation of PKC in COS cells led to activation of Ras and formation of Ras–Raf-1 complexes containing active Raf-1. Raf-1 mutations that prevent its association with Ras blocked activation of Raf-1 by PKC. However, the activation of Raf-1 by PKC was not blocked by dominant negative Ras, indicating that PKC activates Ras by a mechanism distinct from that initiated by activation of receptor tyrosine kinases.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Olson, Professor Michael
• Authors: Marais, R., Light, Y., Mason, C., Paterson, H., Olson, M. F., and Marshall, C. J.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Science
• Publisher: American Association for the Advancement of Science
• ISSN: 0036-8075
• ISSN (Online): 1095-9203