de Vries, S. P.W. et al. (2017) Analysis of Campylobacter jejuni infection in the gnotobiotic piglet and genome-wide identification of bacterial factors required for infection. Scientific Reports, 7, 44283. (doi: 10.1038/srep44283) (PMID:28281647) (PMCID:PMC5345035)
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Abstract
To investigate how Campylobacter jejuni causes the clinical symptoms of diarrhoeal disease in humans, use of a relevant animal model is essential. Such a model should mimic the human disease closely in terms of host physiology, incubation period before onset of disease, clinical signs and a comparable outcome of disease. In this study, we used a gnotobiotic piglet model to study determinants of pathogenicity of C. jejuni. In this model, C. jejuni successfully established infection and piglets developed an increased temperature with watery diarrhoea, which was caused by a leaky epithelium and reduced bile re-absorption in the intestines. Further, we assessed the C. jejuni genes required for infection of the porcine gastrointestinal tract utilising a transposon (Tn) mutant library screen. A total of 123 genes of which Tn mutants showed attenuated piglet infection were identified. Our screen highlighted a crucial role for motility and chemotaxis, as well as central metabolism. In addition, Tn mutants of 14 genes displayed enhanced piglet infection. This study gives a unique insight into the mechanisms of C. jejuni disease in terms of host physiology and contributing bacterial factors.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Everest, Dr Paul and Douce, Dr Gillian and Linn, Dr Aileen and Dagleish, Dr Mark |
| Authors: | de Vries, S. P.W., Linn, A., Macleod, K., MacCallum, A., Hardy, S. P., Douce, G., Watson, E., Dagleish, M. P., Thompson, H., Stevenson, A., Kennedy, D., Baig, A., Coward, C., Maskell, D. J., Smith, D. G.E., Grant, A. J., and Everest, P. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Scientific Reports |
| Publisher: | Nature Research |
| ISSN: | 2045-2322 |
| ISSN (Online): | 2045-2322 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Scientific Reports 7: 44283 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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