Activation of serum response element-regulated genes by lysophosphatidic acid

Perkins, L. M., Ramirez, F. E., Kumar, C. C., Thomson, F. J. and Clark, M. A. (1994) Activation of serum response element-regulated genes by lysophosphatidic acid. Nucleic Acids Research, 22(3), pp. 450-452. (doi: 10.1093/nar/22.3.450) (PMID:8127683) (PMCID:PMC523602)

Abstract

Lysophosphatldic acid (LPA) is an important constituent of serum and shares its mitogenlc activity. Serum induction of several genes is regulated, at least in part, by sequences related to the c-fos serum response element (SRE). A Rat-2 flbroblast cell line containing the β-galactosidase reporter gene under SRE control was treated with LPA. Lysophosphatldic acid induced a time- and dose-dependent increase in β-galactosldase activity. After 5 hours of treatment with 1-oleoyl-LPA a 3-fold increase in β-galactosidase activity was observed. In contrast, endogenous alkaline phosphatase activity did not change in parallel with the /3-galactosidase activity indicating that the induction was specific. Various LPAs with different acyl groups in the sn-1 position induced β-galactosidase activity with a rank order potency of 1-oleoyl-LPA > 1-palmitoyl-LPA ≥ 1-myrlstoyl-LPA > 1-stearoyl-LPA. Phosphatldic acid was approximately equal to 1-stearoyl-LPA. Neither the calcium ionophore (A23187) nor 12-O-tetradecanoylphorbol 13-acetate, Induced β-galactosidase activity. These data suggest that LPA may exert some of its effects by regulation of SRE controlled genes.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Thomson, Dr Fiona
• Authors: Perkins, L. M., Ramirez, F. E., Kumar, C. C., Thomson, F. J., and Clark, M. A.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Nucleic Acids Research
• Publisher: Oxford University Press
• ISSN: 0305-1048
• ISSN (Online): 1362-4962