Wilson, G., Hecht, L. and Barron, L. D. (1997) Evidence for a new cooperative transition in native lysozyme from temperature-dependent Raman optical activity. Journal of Physical Chemistry B, 101(4), pp. 694-698. (doi: 10.1021/jp962480f)
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Abstract
On account of its special sensitivity to conformational mobility in chiral molecular structure, vibrational Raman optical activity has revealed what appears to be a new type of sharp cooperative transition in a native protein, possibly related to its function, from measurements on hen egg white lysozyme in the temperature range 2−50 °C. It occurs at ∼12 °C and involves the loss of residual mobility in tertiary loop and secondary structure and, in tryptophan side groups, in both domains of the protein including the active site region. No significant conformational change appears to be involved. Since differential scanning calorimetry failed to reveal any latent heat, it does not appear to be a simple first-order phase transition.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Hecht, Dr Lutz and Barron, Professor Laurence |
| Authors: | Wilson, G., Hecht, L., and Barron, L. D. |
| College/School: | College of Science and Engineering > School of Chemistry |
| Journal Name: | Journal of Physical Chemistry B |
| Publisher: | American Chemical Society |
| ISSN: | 1520-6106 |
| ISSN (Online): | 1520-5207 |
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