Teraoka, J., Bell, A. F., Hecht, L. and Barron, L. D. (1998) Loop structure in human serum albumin from Raman optical activity. Journal of Raman Spectroscopy, 29(1), pp. 67-71. (doi: 10.1002/(SICI)1097-4555(199801)29:1<67::AID-JRS207>3.0.CO;2-U)
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Abstract
Vibrational Raman optical activity (ROA) measurements on human serum albumin are reported and discussed. At neutral pH, the ROA spectrum in the normal state N is dominated by bands assigned to α-helix together with a strong positive ROA band at ca. 1340 cm-1 assigned to a loop structure with local order possibly that of a 310-helix. On reducing the pH to 3.4, which generates the molten globule-like F state, only small decreases in the α-helix ROA bands occur, but the ca. 1340 cm-1 band decreases by ca. 40%, reflecting a loss of rigidity of part of the loop structure, possibly involving residues 292–315 since these connect the two halves of the molecule thought to be dissociated in the F state. These results suggest that the long loops comprising residues 106–119, 292–315 and 492–511 which connect subdomains A and B within each of the three domains I, II and III and previously described from x-ray studies simply as extended polypeptide might in fact have local order of a 310-helix.
Item Type: | Articles |
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Additional Information: | We thank the Biotechnology and Biological Sciences Research Council for a Research Grant and the Engineering and Physical Sciences Research Council for a Senior Fellowship for L.D.B. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Hecht, Dr Lutz and Barron, Professor Laurence |
Authors: | Teraoka, J., Bell, A. F., Hecht, L., and Barron, L. D. |
College/School: | College of Science and Engineering > School of Chemistry |
Journal Name: | Journal of Raman Spectroscopy |
Publisher: | Wiley |
ISSN: | 0377-0486 |
ISSN (Online): | 1097-4555 |
Published Online: | 04 December 1998 |
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