New insight into the pH-dependent conformational changes in bovine β-lactoglobulin from Raman optical activity

Blanch, E. W., Hecht, L. and Barron, L. D. (1999) New insight into the pH-dependent conformational changes in bovine β-lactoglobulin from Raman optical activity. Protein Science, 8(6), pp. 1362-1367. (doi:10.1110/ps.8.6.1362) (PMID:10386887) (PMCID:PMC2144349)

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Abstract

We have studied the conformation of β-lactoglobulin in aqueous solution at room temperature over the pH range ∼2.0–9.0 using vibrational Raman optical activity (ROA). The ROA spectra clearly show that the basic up and down β-barrel core is preserved over the entire pH range, in agreement with other studies. However, from the shift of a sharp positive ROA band at ∼1268 to ∼1294 cm−1 on going from pH values below that of the Tanford transition, which is centered at pH ∼7.5, to values above, the Tanford transition appears to be associated with changes in the local conformations of residues in loop sequences possibly corresponding to a migration into the α-helical region of the Ramachandran surface from a nearby region. These changes may be related to those detected in X-ray crystal structures which revealed that the Tanford transition is associated with conformational changes in loops which form a doorway to the interior of the protein. The results illustrate how the ability of ROA to detect loop and turn structure separately from secondary structure is useful for studying conformational plasticity in proteins.

Item Type:Articles
Additional Information:We thank the Biotechnology and Biological Sciences Research Council for a Research Grant and the Engineering and Physical Sciences Research Council for a Senior Fellowship for L.D.B.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hecht, Dr Lutz and Barron, Professor Laurence
Authors: Blanch, E. W., Hecht, L., and Barron, L. D.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Protein Science
Publisher:Wiley
ISSN:0961-8368
ISSN (Online):1469-896X

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