Raman optical activity instrument for studies of biopolymer structure and dynamics

Hecht, L., Barron, L. D., Blanch, E. W., Bell, A. F. and Day, L. A. (1999) Raman optical activity instrument for studies of biopolymer structure and dynamics. Journal of Raman Spectroscopy, 30(9), pp. 815-825. (doi:10.1002/(SICI)1097-4555(199909)30:9<815::AID-JRS453>3.0.CO;2-1)

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Abstract

The latest version of a multi-channel Raman optical activity (ROA) instrument implementing incident circular polarization (ICP) modulation within a in a backscattering geometry and optimized for measurements on biopolymers in aqueous solution is described. It is based on a fast single-stage, imaging (stigmatic) monochromator equipped with a high optical density holographic notch filter, a holographic transmission grating and a thinned back-illuminated thermoelectrically cooled charge-coupled device (CCD) detector with a high quantum efficiency. A large-aperture longitudinal electro-optic modulator (Pockels cell) is employed to switch between orthogonal circular polarization states in the incident laser radiation. A thick Lyot depolarizer is used for depolarization of the backscattered Raman radiation. This backscattering ICP CCD ROA design is currently realized with two prototype instruments dedicated to studies of the structure and dynamics of biopolymers in aqueous solution. Backscattered ICP ROA spectra of the following samples are presented as typical examples of the excellent performance characteristics: poly(L-glutamic acid) in α-helical and disordered conformations; bovine α-lactalbumin in native and acid molten globule states; human immunoglobulin; calf thymus DNA and both magnesium-bound and magnesium-free phenylalanine-specific transfer RNA; and filamentous bacterial viruses Pf1 and M13.

Item Type:Articles
Additional Information:We thank the Biotechnology and Biological Sciences Research Council for research grants to L.D.B. and L.H., the Engineering and Physical Sciences Research Council for a Senior Fellowship to L.D.B. and the Public Health Research Institute for support of L.A.D.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hecht, Dr Lutz and Barron, Professor Laurence
Authors: Hecht, L., Barron, L. D., Blanch, E. W., Bell, A. F., and Day, L. A.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Journal of Raman Spectroscopy
Publisher:Wiley
ISSN:0377-0486
ISSN (Online):1097-4555
Published Online:27 August 1999

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