Low-affinity NGF-receptor and E-N-CAM expression define two types of olfactory nerve ensheathing cells that share a common lineage

Franceschini, I. A. and Barnett, S. C. (1996) Low-affinity NGF-receptor and E-N-CAM expression define two types of olfactory nerve ensheathing cells that share a common lineage. Developmental Biology, 173(1), pp. 327-343. (doi:10.1006/dbio.1996.0027) (PMID:8575633)

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Abstract

Previously, we have shown that the O4 antibody can be used to define and purify olfactory nerve ensheathing cells (ONECs) from the rat olfactory bulb by fluorescence-activated cell sorting. In this study, using a larger panel of neural markers, we demonstrate that this apparently homogeneous population of ONECs possess a heterogeneous antigenic profile bothin vivoandin vitro.The antigenic profile of the sorted cells initially correlated with their antigenic profilein vivo,although expression of some of the markers was either lost or gained during time in culture. These changes were influenced by the culture conditions, with a greater loss of “typical” ONEC markers in serum-containing medium. In serum-free medium, which maintains the cells in a phenotype that closely resembles theirin vivocounterparts, we were able to reclassify the ONECs into two cell types based on morphology and antigenic phenotype by using antibodies to polysialic acid (correlating with the embryonic form of N-CAM expression) and the low-affinity nerve growth factor receptor. A detailed immunocytochemical study of the developing olfactory system showed that these two cell types could also be detected along the entire length of the olfactory nerve and the outer layer of the olfactory bulb from Embryonic Day 14 to adulthood, suggesting they were not anin vitroartefact. To address the relationship between the two cell types we constructed a clonal ONEC cell line by retroviral infection with the temperature-sensitive mutant gene of the large T antigen. This clonal cell line contained cells that expressed antigenic phenotypes of both classes of ONECs, suggesting that both cell types are related and share a common lineage.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Barnett, Professor Susan
Authors: Franceschini, I. A., and Barnett, S. C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Developmental Biology
Publisher:Elsevier
ISSN:0012-1606
ISSN (Online):1095-564X
Published Online:07 May 2002

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