Pinto, S. B., Mariconti, M., Bazzocchi, C., Bandi, C. and Sinkins, S. P. (2012) Wolbachia surface protein induces innate immune responses in mosquito cells. BMC Microbiology, 12(Suppl), S11. (doi: 10.1186/1471-2180-12-S1-S11) (PMID:22375833) (PMCID:PMC3287508)
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Abstract
Background: Wolbachiaendosymbiotic bacteria are capable of inducing chronic upregulation of insect immune genes in some situations and this phenotype may influence the transmission of important insect-borne pathogens. However the molecules involved in these interactions have not been characterized. Results: Here we show that recombinant Wolbachia Surface Protein (WSP) stimulates increased transcription of immune genes in mosquito cells derived from the mosquito Anopheles gambiae, which is naturally uninfected with Wolbachia; at least two of the upregulated genes, TEP1 and APL1, are known to be important in Plasmodium killing in this species. When cells from Aedes albopictus, which is naturally Wolbachia-infected, were challenged with WSP lower levels of upregulation were observed than for the An. gambiaecells. Conclusions: We have found that WSP is a strong immune elicitor in a naturally Wolbachia-uninfected mosquito species (Anopheles gambiae) while a milder elicitor in a naturally-infected species (Aedes albopictus). Since the WSP of a mosquito non-native (nematode) Wolbachia strain was used, these data suggest that there is a generalized tolerance to WSP in Ae. albopictus.
Item Type: | Articles |
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Additional Information: | This study was supported by the Wellcome Trust (grant number 079059) and by MIUR-PRIN 2009. MM received support from EU-COST Action FA0701. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Sinkins, Professor Steven |
Authors: | Pinto, S. B., Mariconti, M., Bazzocchi, C., Bandi, C., and Sinkins, S. P. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | BMC Microbiology |
Publisher: | BioMed Central |
ISSN: | 1471-2180 |
ISSN (Online): | 1471-2180 |
Copyright Holders: | Copyright © 2012 Pino et al. |
First Published: | First published in BMC Microbiology 12(Suppl 1): S11 |
Publisher Policy: | Reproduced under a Creative Commons License |
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