Activation of ion channels in tumor cells by leukoregulin, a cytostatic lymphokin

Sheehy, P.A., Barnett, S.C. , Evans, C.H. and Barker, J.L. (1988) Activation of ion channels in tumor cells by leukoregulin, a cytostatic lymphokin. Journal of the National Cancer Institute, 80(11), pp. 868-871. (doi: 10.1093/jnci/80.11.868) (PMID:2455812)

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We used the whole-cell patch-clamp recording technique to study the effects of leukoregulin (LR), a cytostatic lymphokine produced by activated human peripheral blood lymphocytes, on the electrical properties of K562 tumor cells. LR induced changes in the membrane excitability in 33 of 55 cells studied. A minute or more after application, LR elicited a complex and reversible electrical response. The response lasted for several more minutes in the continued presence of LR. It consisted of (a) moderate-conductance (50 pS), cation-selective, ion-channel activity, (b) a shift of the zero-current membrane potential close to 0 mV, and (c) a suppression of a depolarizatlon-activated K* conductance. All of these changes in tumor cell excitability act coordinately to depolarize the tumor cells and may be important in the cytostatic actions of LR.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Barnett, Professor Susan
Authors: Sheehy, P.A., Barnett, S.C., Evans, C.H., and Barker, J.L.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Journal of the National Cancer Institute
Publisher:Oxford University Press
ISSN (Online):1460-2105

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