SCA-1 labels a subset of estrogen-responsive bipotential repopulating cells within the CD24(+) CD49f(hi) mammary stem cell-enriched compartment

Dall, G. V. et al. (2017) SCA-1 labels a subset of estrogen-responsive bipotential repopulating cells within the CD24(+) CD49f(hi) mammary stem cell-enriched compartment. Stem Cell Reports, 8(2), pp. 417-431. (doi: 10.1016/j.stemcr.2016.12.022) (PMID:28132885)

Abstract

Estrogen stimulates breast development during puberty and mammary tumors in adulthood through estrogen receptor-α (ERα). These effects are proposed to occur via ERα+ luminal cells and not the mammary stem cells (MaSCs) that are ERαneg. Since ERα+ luminal cells express stem cell antigen-1 (SCA-1), we sought to determine if SCA-1 could define an ERα+ subset of EpCAM+/CD24+/CD49fhi MaSCs. We show that the MaSC population has a distinct SCA-1+ population that is abundant in pre-pubertal mammary glands. The SCA-1+ MaSCs have less stem cell markers and less in vivo repopulating activity than their SCA-1neg counterparts. However, they express ERα and specifically enter the cell cycle at puberty. Using estrogen-deficient aromatase knockouts (ArKO), we showed that the SCA-1+ MaSC could be directly modulated by estrogen supplementation. Thus, SCA-1 enriches for an ERα+, estrogen-sensitive subpopulation within the CD24+/CD49fhi MaSC population that may be responsible for the hormonal sensitivity of the developing mammary gland.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Stein, Dr Torsten and Morris, Professor Joanna
• Authors: Dall, G. V., Vieusseux, J. L., Korach, K. S., Arao, Y., Hewitt, S. C., Hamilton, K. J., Dzierzak, E., Boon, W. C., Simpson, E. R., Ramsay, R. G., Stein, T., Morris, J. S., Anderson, R. L., Risbridger, G. P., and Britt, K. L.
• Subjects: ?? Sca-1 ??; ?? estrogen ??; ?? mammary stem cells ??
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences; College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Stem Cell Reports
• Publisher: Elsevier (Cell Press)
• ISSN: 2213-6711
• ISSN (Online): 2213-6711
• Published Online: 26 January 2017
• Copyright Holders: Copyright © 2017 The Authors
• First Published: First published in Stem Cell Reports 8(2):417-431
• Publisher Policy: Reproduced under a Creative Commons License