Isolation and differentiation of human macrophages

Rios, F. J. , Touyz, R. M. and Montezano, A. C. (2017) Isolation and differentiation of human macrophages. In: Touyz, R. M. and Schiffrin, E. L. (eds.) Hypertension: Methods and Protocols. Series: Methods in molecular biology, 1527. Springer: New York, pp. 311-320. ISBN 9781493966233 (doi: 10.1007/978-1-4939-6625-7_24)

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Macrophage subtypes display protective or pathogenic activities in vascular lesions. They recognize and engulf modified lipids, accumulate in the arterial intima, contributing to the atherosclerosis development. In the heart, vascular tissues and perivascular adipose tissues, there is increasing evidence that macrophages play a role in endothelial dysfunction, vascular inflammation and remodeling. In this chapter we describe protocols to isolate human monocytes from peripheral blood mononuclear cell and how to differentiate them into macrophages by using growth factors (granulocyte-macrophage colony-stimulating factor—GM-CSF or colony-stimulating factor—M-CSF) or by different cell culture medium concentration. We also describe how to indentify different macrophage activation phenotypes.

Item Type:Book Sections
Glasgow Author(s) Enlighten ID:Rios, Dr Francisco and Montezano, Dr Augusto and Touyz, Professor Rhian
Authors: Rios, F. J., Touyz, R. M., and Montezano, A. C.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
Published Online:24 January 2017

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