Reclassification of Bifidobacterium stercoris Kim et al. 2010 as a later heterotypic synonym of Bifidobacterium adolescentis

Killer, J., Sedlacek, I., Rada, V., Havlik, J. and Kopecny, J. (2013) Reclassification of Bifidobacterium stercoris Kim et al. 2010 as a later heterotypic synonym of Bifidobacterium adolescentis. International Journal of Systematic and Evolutionary Microbiology, 63(Pt 11), pp. 4350-4353. (doi: 10.1099/ijs.0.054957-0) (PMID:24187022)

Abstract

The taxonomic position of Bifidobacterium stercoris Eg1T ( = JCM 15918T) based on comparative 16S rRNA gene and hsp60 sequence analyses was found to be controversial, as the strain showed high similarity to the type strain of Bifidobacterium adolescentis , CCUG 18363T. Therefore, the relationship between the two species was investigated by a taxonomic study that included, in addition to re-evaluation of the 16S rRNA gene sequence, determination of DNA–DNA binding and multilocus sequence analysis (MLSA) of housekeeping genes encoding the DNA-directed RNA polymerase B subunit (rpoC), putative xylulose-5-phosphate/fructose-6-phosphate phosphoketolase (xfp), elongation factor EF-G (fusA), 50S ribosomal protein L2 (rplB) and DNA gyrase B subunit (gyrB). Comparative 16S rRNA gene sequence analysis showed relatively high similarity (98.9 %) between B. stercoris KCTC 5756T and B. adolescentis ATCC 15703T. MLSA revealed close relatedness between B. stercoris KCTC 5756T and B. adolescentis CCUG 18363T, with 99.3–100 % similarity between the rpoC, xfp, fusA, rplB and gyrB gene sequences. In addition, relatively high dnaJ1 gene sequence similarity of 97.7 % was found between the strains. Similar phenotypes and a high DNA–DNA binding value (78.9 %) confirmed that B. stercoris and B. adolescentis are synonymous. Based on these results, it is proposed that the species Bifidobacterium stercoris Kim et al. 2010 should be reclassified as a later heterotypic synonym of Bifidobacterium adolescentis Reuter 1963 (Approved Lists 1980).

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Havlik, Dr Jaroslav
• Authors: Killer, J., Sedlacek, I., Rada, V., Havlik, J., and Kopecny, J.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
• Journal Name: International Journal of Systematic and Evolutionary Microbiology
• Publisher: Microbiology Society
• ISSN: 1466-5026
• ISSN (Online): 1466-5034
• Published Online: 01 November 2013