Cytotoxic constituents of Pachyrhizus tuberosus from Peruvian Amazon

Leuner, O. et al. (2013) Cytotoxic constituents of Pachyrhizus tuberosus from Peruvian Amazon. Natural Product Communications, 8(10), pp. 1423-1426. (PMID:24354192)

Abstract

Investigations into the chemical constituents of the seeds of the neglected tuber crop Pachyrhizus tuberosus (Leguminosae) resulted in the isolation of seven components: five rotenoids [12a-hydroxyerosone (1), 12a-hydroxydolineone (2), erosone (3), 12a-hydroxyrotenone (4) and rotenone (6)], a phenylfuranocoumarin [pachyrrhizine (5)] and an isoflavanone [neotenone (7)]. The compounds were isolated using several chromatography techniques and characterized and verified by NMR and HPLC/MS. The MTT assay was used to examine the selective cytotoxic effects of the methanolic P. tuberosus extract and isolated compounds in two human cancer cell lines [breast (MCF-7) and colorectal (HCT-116)] and in non-transformed human fibroblasts (MRC-5); IC50 values were calculated. The methanolic P. tuberosus extract displayed respectable cytotoxic effects against HCT-116 and MCF-7 cells with IC50 values of 7.3 and 6.3 mu g/mL, respectively. Of the compounds, 6 exacted greatest cytotoxicity and selectivity towards the cancer cell lines tested, yielding IC50 values of 0.3 mu g/mL against both MCF-7 and HCT-116 cells, and a 6-fold reduced activity against MRC-5 fibroblasts. Compound 4 also demonstrated cytotoxicity against MCF-7 and HCT-116 (1.1 and 1.8 mu g/mL, respectively), and reduced cytotoxicity towards MRC-5 cells (7.5 mu g/mL). The results revealed from the in vitro cytotoxic MTT assay are worthy of further antitumor investigation.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Havlik, Dr Jaroslav
• Authors: Leuner, O., Havlik, J., Budesinsky, M., Vrkoslav, V., Chu, J., Bradshaw, T.D., Hummelova, J., Miksatkova, P., Lapcik, O., Valterova, C., and Kokosa, L.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
• Journal Name: Natural Product Communications
• Publisher: Natural Product Incorporation
• ISSN: 1934-578X
• ISSN (Online): 1555-9475