Volštátová, T., Havlik, J. , Doskočil, I., Geigerová, M. and Rada, V. (2015) Effect of hydrolyzed milk on the adhesion of Lactobacilli to intestinal cells. Scientia Agriculturae Bohemica, 46(1), pp. 21-25. (doi: 10.1515/sab-2015-0012)
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Abstract
Milk is an essential part of the human diet and is undoubtedly a major calcium source in human nutrition, accepted well by most individuals. Knowledge on how the components from dairy products support or reduce the adherence of probiotics to the intestinal epithelium is limited. The purpose of this study was to investigate the effect of acid-hydrolyzed milk on the adhesion ability of two potentially probiotic strains (Lactobacillus plantarum S2, Lactobacillus gasseri R) to in vitro human intestinal epithelial model consisting of Caco-2 and mucus-secreting HT29-MTX co-culture. The adhesion of our tested strains L. gasseri and L. plantarum was 4.74 and 7.16%, respectively, when using inoculum of 2 × 108 CFU ml–1. Addition of acid-hydrolyzed milk to co-culture decreased the adherence by 53.7% for L. gasseri R and by 62.2% for L. plantarum S2. The results of this study evidently indicate the potential importance of the food matrix as a factor influencing probiotic colonization of the gut.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Havlik, Dr Jaroslav |
Authors: | Volštátová, T., Havlik, J., Doskočil, I., Geigerová, M., and Rada, V. |
College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
Journal Name: | Scientia Agriculturae Bohemica |
Publisher: | De Gruyter |
ISSN: | 1211-3174 |
ISSN (Online): | 1805-9430 |
Published Online: | 04 April 2015 |
Copyright Holders: | Copyright © 2015 T. Volštátová et al. |
First Published: | First published in Scientia Agriculturae Bohemica 46(1):21-25 |
Publisher Policy: | Reproduced under a creative commons license |
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