Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases

Colloms, S. D. , Sykora, P., Szatmari, G. and Sherratt, D. J. (1990) Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases. Journal of Bacteriology, 172(12), pp. 6973-6980. (doi: 10.1128/jb.172.12.6973-6980.1990) (PMID:2254268) (PMCID:PMC210817)

Abstract

Site-specific recombination at the plasmid ColE1 cer site requires the Escherichia coli chromosomal gene xerC. The xerC gene has been localized to the 85-min region of the E. coli chromosome, between cya and uvrD. The nucleotide sequences of the xerC gene and flanking regions have been determined. The xerC gene encodes a protein with a calculated molecular mass of 33.8 kDa. This protein has substantial sequence similarity to the lambda integrase family of site-specific recombinases and is probably the cer recombinase. The xerC gene is expressed as part of a multicistronic unit that includes the dapF gene and two other open reading frames.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Colloms, Dr Sean
• Authors: Colloms, S. D., Sykora, P., Szatmari, G., and Sherratt, D. J.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Journal of Bacteriology
• Publisher: American Society for Microbiology
• ISSN: 0021-9193
• ISSN (Online): 1098-5530