DNA binding activities of the Caenorhabditis elegans Tc3 transposase

Colloms, S. D. , van Luenen, H. G.A.M. and PIasterk, R. H.A. (1994) DNA binding activities of the Caenorhabditis elegans Tc3 transposase. Nucleic Acids Research, 22(25), pp. 5548-5554. (doi: 10.1093/nar/22.25.5548) (PMID:7838706) (PMCID:PMC310115)

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Tc3 Is a member of the Tc1/mariner family of transposable elements. All these elements have terminal Inverted repeats, encode related transposases and Insert exclusively into TA dinucleotides. We have studied the DNA binding properties of Tc3 transposase and found that an N-termlnal domain of 65 amino acids binds specifically to two regions within the 462 bp Tc3 inverted repeat; one region is located at the end of the inverted repeat, the other is located ∼ 180 bp from the end. Methylatlon interference experiments indicate that this N-termlnal DNA binding domain of the Tc3 transposase Interacts with nucleotides on one face of the DNA helix over adjacent major and minor grooves.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Colloms, Dr Sean
Authors: Colloms, S. D., van Luenen, H. G.A.M., and PIasterk, R. H.A.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Nucleic Acids Research
Publisher:Oxford University Press
ISSN (Online):1362-4962

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