Assay Method

Reactivlab Ltd (2013) Assay Method. .

Abstract

The invention provides an assay method for determining a level of haptoglobin in a sample comprising the steps of: (i) mixing haemoglobin with the sample to be assayed so as to form a haptoglobin-haemoglobin complex with haptoglobin present in the sample: (ii) contacting the product of step (i) with reagents for generating hydrogen peroxide and one or more chromogens which undergo a spectroscopically detectable change when peroxidase activity is present, in the presence of a buffer, under condition in which hydrogen peroxide is generated from said reagents and forms a substrate for the peroxidise activity of the haptoglobin-haemoglobin complex present, and wherein the pH of the buffer is within a range which is sufficiently low that the peroxidise activity of any uncomplexed haemoglobin is substantially suppressed but sufficiently high that hydrogen peroxide generation occurs: (iii) determining the peroxidase activity of the haptoglobin-haemoglobin complex by measuring the change in an optical property of the reaction mixture: and (iv) correlating the level of peroxidise activity of the haptoglobin-haemoglobin complex with the amount of haptoglobin in the sample. A kit for use in such a method is also provided.

• Item Type: Patents
• Status: Published
• Glasgow Author(s) Enlighten ID: Eckersall, Professor David and Mcculloch, Miss Eilidh
• Authors: Eckersall, P. D., Mcculloch, E., and Docherty, S.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences