Ling, I. T.C., Rochard, L. and Liao, E. C. (2017) Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification. Developmental Biology, 421(2), pp. 219-232. (doi: 10.1016/j.ydbio.2016.11.016) (PMID:27908786) (PMCID:PMC5266562)
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Abstract
Formation of the mandible requires progressive morphologic change, proliferation, differentiation and organization of chondrocytes preceding osteogenesis. The Wnt signaling pathway is involved in regulating bone development and maintenance. Chondrocytes that are fated to become bone require Wnt to polarize and orientate appropriately to initiate the endochondral ossification program. Although the canonical Wnt signaling has been well studied in the context of bone development, the effects of non-canonical Wnt signaling in regulating the timing of cartilage maturation and subsequent bone formation in shaping ventral craniofacial structure is not fully understood.. Here we examined the role of the non-canonical Wnt signaling pathway (wls, gpc4, wnt5b and wnt9a) in regulating zebrafish Meckel's cartilage maturation to the onset of osteogenic differentiation. We found that disruption of wls resulted in a significant loss of craniofacial bone, whereas lack of gpc4, wnt5b and wnt9a resulted in severely delayed endochondral ossification. This study demonstrates the importance of the non-canonical Wnt pathway in regulating coordinated ventral cartilage morphogenesis and ossification.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by National Institute of Dental and Craniofacial Research Grant R03DE024490 and by a grant from Shriners Hospital for Children to ECL. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ling, Dr Irving |
| Authors: | Ling, I. T.C., Rochard, L., and Liao, E. C. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
| Journal Name: | Developmental Biology |
| Publisher: | Elsevier |
| ISSN: | 0012-1606 |
| ISSN (Online): | 1095-564X |
| Published Online: | 29 November 2016 |
| Copyright Holders: | Copyright © 2016 The Authors |
| First Published: | First published in Developmental Biology 421(2): 219-232 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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