Implantation of a poly-L-lactide GCSF-functionalized scaffold in a model of chronic myocardial infarction

Spadaccio, C. et al. (2017) Implantation of a poly-L-lactide GCSF-functionalized scaffold in a model of chronic myocardial infarction. Journal of Cardiovascular Translational Research, 10(1), pp. 47-65. (doi: 10.1007/s12265-016-9718-9) (PMID:28116550) (PMCID:PMC5323505)

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Abstract

A previously developed poly-l-lactide scaffold releasing granulocyte colony-stimulating factor (PLLA/GCSF) was tested in a rabbit chronic model of myocardial infarction (MI) as a ventricular patch. Control groups were constituted by healthy, chronic MI and nonfunctionalized PLLA scaffold. PLLA-based electrospun scaffold efficiently integrated into a chronic infarcted myocardium. Functionalization of the biopolymer with GCSF led to increased fibroblast-like vimentin-positive cellular colonization and reduced inflammatory cell infiltration within the micrometric fiber mesh in comparison to nonfunctionalized scaffold; PLLA/GCSF polymer induced an angiogenetic process with a statistically significant increase in the number of neovessels compared to the nonfunctionalized scaffold; PLLA/GCSF implanted at the infarcted zone induced a reorganization of the ECM architecture leading to connective tissue deposition and scar remodeling. These findings were coupled with a reduction in end-systolic and end-diastolic volumes, indicating a preventive effect of the scaffold on ventricular dilation, and an improvement in cardiac performance.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Spadaccio, Dr Cristiano
Authors: Spadaccio, C., Nappi, F., De Marco, F., Sedati, P., Taffon, C., Nenna, A., Crescenzi, A., Chello, M., Trombetta, M., Gambardella, I., and Rainer, A.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
Journal Name:Journal of Cardiovascular Translational Research
Publisher:Springer Verlag
ISSN:1937-5387
ISSN (Online):1937-5395
Published Online:23 January 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Journal of Cardiovascular Translational Research 10(1):47-65
Publisher Policy:Reproduced under a Creative Commons License

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