Tyrrell, B. J., Woodham, E. F., Spence, H. J., Strathdee, D., Insall, R. H. and Machesky, L. M. (2016) Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. Pigment Cell and Melanoma Research, 29(5), pp. 559-571. (doi: 10.1111/pcmr.12506) (PMID:27390154) (PMCID:PMC5082549)
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Abstract
The five-subunit WASH complex generates actin networks that participate in endocytic trafficking, migration and invasion in various cell types. Loss of one of the two subunits WASH or strumpellin in mice is lethal, but little is known about their role in mammals in vivo. We explored the role of strumpellin, which has previously been linked to hereditary spastic paraplegia, in the mouse melanocytic lineage. Strumpellin knockout in melanocytes revealed abnormal endocytic vesicle morphology but no impairment of migration in vitro or in vivo and no change in coat colour. Unexpectedly, WASH and filamentous actin could still localize to vesicles in the absence of strumpellin, although the shape and size of vesicles was altered. Blue native PAGE revealed the presence of two distinct WASH complexes, even in strumpellin knockout cells, revealing that the WASH complex can assemble and localize to endocytic compartments in cells in the absence of strumpellin.
Item Type: | Articles |
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Additional Information: | The authors acknowledge funding for this project from Cancer Research UK Core funding to LMM (A15673), RHI (A15672) and to the Biological Services Unit (A17196). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Machesky, Professor Laura and Insall, Professor Robert and Strathdee, Mr Douglas and Tyrrell, Mr Benjamin and Spence, Dr Heather |
Authors: | Tyrrell, B. J., Woodham, E. F., Spence, H. J., Strathdee, D., Insall, R. H., and Machesky, L. M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Journal Name: | Pigment Cell and Melanoma Research |
Publisher: | Wiley |
ISSN: | 1755-1471 |
ISSN (Online): | 1755-148X |
Published Online: | 12 September 2016 |
Copyright Holders: | Copyright © 2016 The Authors |
First Published: | First published in Pigment Cell and Melanoma Research 29(5): 559-571 |
Publisher Policy: | Reproduced under a Creative Commons License |
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