Orellana-Tavra, C., Marshall, R. J., Baxter, E. F., Abanades-Lazaro, I., Tao, A., Cheetham, A. K., Forgan, R. S. and Fairen-Jimenez, D. (2016) Drug delivery and controlled release from biocompatible metal-organic frameworks using mechanical amorphization. Journal of Materials Chemistry B, 4(47), pp. 7697-7707. (doi: 10.1039/C6TB02025A)
|
Text
130976.pdf - Published Version Available under License Creative Commons Attribution. 4MB |
Abstract
We have used a family of Zr-based metal-organic frameworks (MOFs) with different functionalized (bromo, nitro and amino) and extended linkers for drug delivery. We loaded the materials with the fluorescent model molecule calcein and the anticancer drug α-cyano-4-hydroxycinnamic acid (α-CHC), and consequently performed a mechanical amorphization process to attempt to control the delivery of guest molecules. Our analysis revealed that the loading values of both molecules were higher for the MOFs containing unfunctionalized linkers. Confocal microscopy showed that all the materials were able to penetrate into cells, and the therapeutic effect of α-CHC on HeLa cells was enhanced when loaded (20 wt.%) into the MOF with the longest linker. On the one hand, calcein release required up to 3 days from the crystalline form for all the materials. On the other hand, the amorphous counterparts containing the bromo and nitro functional groups released only a fraction of the total loaded amount, and in the case of the amino-MOF a slow and progressive release was successfully achieved for 15 days. In the case of the materials loaded with α-CHC, no difference was observed between the crystalline and amorphous form of the materials. These results highlight the necessity of a balance between the pore size of the materials and the size of the guest molecules to accomplish a successful and efficient sustained release using this mechanical ball-milling process. Additionally, the endocytic pathway used by cells to internalize these MOFs may lead to diverse final cellular locations and consequently, different therapeutic effects. Understanding these cellular mechanisms will drive the design of more effective MOFs for drug delivery applications.
| Item Type: | Articles |
|---|---|
| Additional Information: | C. A. O. thanks Becas Chile and the Cambridge Trust for funding. D. F.-J. thanks the Royal Society (UK) for funding through a University Research Fellowship. RSF thanks the Royal Society for receipt of a University Research Fellowship and the EPSRC (EP/L004461/1) and The University of Glasgow for funding. A.K.C is grateful to the European Research Council for an Advanced Investigator Award. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Forgan, Professor Ross and Marshall, Mr Ross James |
| Authors: | Orellana-Tavra, C., Marshall, R. J., Baxter, E. F., Abanades-Lazaro, I., Tao, A., Cheetham, A. K., Forgan, R. S., and Fairen-Jimenez, D. |
| College/School: | College of Science and Engineering > School of Chemistry |
| Journal Name: | Journal of Materials Chemistry B |
| Publisher: | Royal Society of Chemistry |
| ISSN: | 2050-750X |
| ISSN (Online): | 2050-7518 |
| Published Online: | 03 November 2016 |
| Copyright Holders: | Copyright © 2016 The Authors |
| First Published: | First published in Journal of Materials Chemistry B 4:7697-7707 |
| Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
Funder and Project Information