Dheeman, D. S., Frias, J. M. and Henehan, G. T.M. (2010) Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304. Journal of Industrial Microbiology and Biotechnology, 37(1), pp. 1-17. (doi: 10.1007/s10295-009-0643-7) (PMID:19806375)
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Abstract
Among several lipase-producing actinomycete strains screened, Amycolatopsis mediterranei DSM 43304 was found to produce a thermostable, extracellular lipase. Culture conditions and nutrient source modification studies involving carbon sources, nitrogen sources, incubation temperature and medium pH were carried out. Lipase activity of 1.37 +/- 0.103 IU/ml of culture medium was obtained in 96 h at 28 degrees C and pH 7.5 using linseed oil and fructose as carbon sources and a combination of phytone peptone and yeast extract (5:1) as nitrogen sources. Under optimal culture conditions, the lipase activity was enhanced 12-fold with a twofold increase in lipase specific activity. The lipase showed maximum activity at 60 degrees C and pH 8.0. The enzyme was stable between pH 5.0 and 9.0 and temperatures up to 60 degrees C. Lipase activity was significantly enhanced by Fe(3+) and strongly inhibited by Hg(2+). Li(+), Mg(2+) and PMSF significantly reduced lipase activity, whereas other metal ions and effectors had no significant effect at 0.01 M concentration. A. mediterranei DSM 43304 lipase exhibited remarkable stability in the presence of a wide range of organic solvents at 25% (v/v) concentration for 24 h. These features render this novel lipase attractive for potential biotechnological applications in organic synthesis reactions.
| Item Type: | Articles (Other) |
|---|---|
| Additional Information: | This work was supported by ABBEST Research Scholarship from Dublin Institute of Technology (PB 03557/2007). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Dheeman, Dr Dharmendra |
| Authors: | Dheeman, D. S., Frias, J. M., and Henehan, G. T.M. |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Journal of Industrial Microbiology and Biotechnology |
| Publisher: | Springer |
| ISSN: | 1367-5435 |
| ISSN (Online): | 1476-5535 |
| Published Online: | 06 October 2009 |
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