Jakob, P. H., Kehrer, J., Flood, P., Wiegel, C., Haselmann, U., Meissner, M. , Stelzer, E. H.K. and Reynaud, E. G. (2016) A 3-D cell culture system to study epithelia functions using microcarriers. Cytotechnology, 68(5), pp. 1813-1825. (doi: 10.1007/s10616-015-9935-0) (PMID:26847791)
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Abstract
In vitro cell culture models used to study epithelia and epithelial diseases would benefit from the recognition that organs and tissues function in a three-dimensional (3D) environment. This context is necessary for the development of cultures that more realistically resemble in vivo tissues/organs. Our aim was to establish and characterize biologically meaningful 3D models of epithelium. We engineered 3D epithelia cultures using a kidney epithelia cell line (MDCK) and spherical polymer scaffolds. These kidney epithelia were characterized by live microscopy, immunohistochemistry and transmission electron microscopy. Strikingly, the epithelial cells displayed increased physiological relevance; they were extensively polarized and developed a more differentiated phenotype. Using such a growth system allows for direct transmission and fluorescence imaging with few restrictions using wide-field, confocal and Light Sheet Fluorescence Microscopy. We also assessed the wider relevance of this 3D culturing technique with several epithelial cell lines. Finally, we established that these 3D micro-tissues can be used for infection as well as biochemical assays and to study important cellular processes such as epithelial mesenchymal transmission. This new biomimetic model could provide a broadly applicable 3D culture system to study epithelia and epithelia related disorders.
Item Type: | Articles |
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Additional Information: | This work was funded by BMBF Grant ‘‘QuantPro’’, Number 0313831D. EGR acknowledges support from the SFI under the Stokes Fellowship Programme. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Meissner, Professor Markus |
Authors: | Jakob, P. H., Kehrer, J., Flood, P., Wiegel, C., Haselmann, U., Meissner, M., Stelzer, E. H.K., and Reynaud, E. G. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Cytotechnology |
Publisher: | Springer |
ISSN: | 0920-9069 |
ISSN (Online): | 1573-0778 |
Published Online: | 04 February 2016 |
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