Full genome sequence and sfRNA interferon antagonist activity of Zika virus from Recife, Brazil

Donald, C. L. et al. (2016) Full genome sequence and sfRNA interferon antagonist activity of Zika virus from Recife, Brazil. PLoS Neglected Tropical Diseases, 10(10), e0005048. (doi: 10.1371/journal.pntd.0005048) (PMID:27706161) (PMCID:PMC5051680)

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Background: The outbreak of Zika virus (ZIKV) in the Americas has transformed a previously obscure mosquito-transmitted arbovirus of the Flaviviridae family into a major public health concern. Little is currently known about the evolution and biology of ZIKV and the factors that contribute to the associated pathogenesis. Determining genomic sequences of clinical viral isolates and characterization of elements within these are an important prerequisite to advance our understanding of viral replicative processes and virus-host interactions. Methodology/Principal findings: We obtained a ZIKV isolate from a patient who presented with classical ZIKV-associated symptoms, and used high throughput sequencing and other molecular biology approaches to determine its full genome sequence, including non-coding regions. Genome regions were characterized and compared to the sequences of other isolates where available. Furthermore, we identified a subgenomic flavivirus RNA (sfRNA) in ZIKV-infected cells that has antagonist activity against RIG-I induced type I interferon induction, with a lesser effect on MDA-5 mediated action. Conclusions/Significance: The full-length genome sequence including non-coding regions of a South American ZIKV isolate from a patient with classical symptoms will support efforts to develop genetic tools for this virus. Detection of sfRNA that counteracts interferon responses is likely to be important for further understanding of pathogenesis and virus-host interactions.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Davis, Dr Chris and Selinger, Mr Martin and Wilkie, Dr Gavin and Da Silva Filipe, Dr Ana and Biek, Professor Roman and Brennan, Dr Benjamin and Hughes, Dr Joseph and Donald, Dr Claire and Clark, Mr Jordan and Fall, Dr Gamou and Owsianka, Dr Anna and Schnettler, Dr Esther and Varjak, Dr Margus and Patel, Professor Arvind and Kohl, Professor Alain and McLauchlan, Professor John and Cumberworth, Miss Stephanie
Authors: Donald, C. L., Brennan, B., Cumberworth, S. L., Rezelj, V. V., Clark, J. J., Cordeiro, M. T., Freitas de Oliveira França, R., Pena, L. J., Wilkie, G. S., Da Silva Filipe, A., Davis, C., Hughes, J., Varjak, M., Selinger, M., Zuvanov, L., Owsianka, A. M., Patel, A. H., McLauchlan, J., Lindenbach, B. D., Fall, G., Sall, A. A., Biek, R., Rehwinkel, J., Schnettler, E., and Kohl, A.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:PLoS Neglected Tropical Diseases
Publisher:Public Library of Science
ISSN (Online):1935-2735
Copyright Holders:Copyright © 2016 Donald et al.
First Published:First published in PLoS Neglected Tropical Diseases 10(10:e0005048
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656321Genomics of human cytomegalovirusAndrew DavisonMedical Research Council (MRC)MC_UU_12014/3MVLS III - CENTRE FOR VIRUS RESEARCH
708681The emergence of Zika virus in Brazil: investigating prevalence and host responses to design preventive strategiesAlain KohlMedical Research Council (MRC)MR/N017552/1MVLS III - CENTRE FOR VIRUS RESEARCH