Recent advances in liquid and gas chromatography methodology for extending coverage of the metabolome

Haggarty, J. and Burgess, K. E.V. (2017) Recent advances in liquid and gas chromatography methodology for extending coverage of the metabolome. Current Opinion in Biotechnology, 43, pp. 77-85. (doi: 10.1016/j.copbio.2016.09.006) (PMID:27771607)

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The metabolome is the complete complement of metabolites (small organic biomolecules). In order to comprehensively understand the effect of stimuli on a biological system, it is important to detect as many of the metabolites within that system as possible. This review briefly describes some new advances in liquid and gas chromatography to improve coverage of the metabolome, including the serial combination of two columns in tandem, column switching and different variations of two-dimensional chromatography. Supercritical fluid chromatography could provide complimentary data to liquid and gas chromatography. Although there have been many recent advancements in the field of metabolomics, it is evident that a combination, rather than a single method, is required to approach full coverage of the metabolome.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Burgess, Dr Karl and Haggarty, Ms Jennifer
Authors: Haggarty, J., and Burgess, K. E.V.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Current Opinion in Biotechnology
ISSN (Online):1879-0429
Published Online:20 October 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Current Opinion in Biotechnology 43:77-85
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
470561DTC in cell and proteomic technologies (continuation)Jonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/F500424/1ENG - BIOMEDICAL ENGINEERING
593571Manipulation of cancer cells by nanotopography: strategies to control migration, proliferation and apoptosis (ISSF Catalyst Fund)Penelope TsimbouriWellcome Trust (WELLCOME)097821/Z/11/ZRI MOLECULAR CELL & SYSTEMS BIOLOGY
623593Institutional Strategic Support Fund (ISSF)Anna DominiczakWellcome Trust (WELLCOME)105614/Z/14/ZRI CARDIOVASCULAR & MEDICAL SCIENCES