Bayes, H.K., Ritchie, N., Irvine, S. and Evans, T.J. (2016) A murine model of early Pseudomonas aeruginosa lung disease with transition to chronic infection. Scientific Reports, 6, 35838. (doi: 10.1038/srep35838) (PMID:27804985) (PMCID:PMC5090221)
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Abstract
Pseudomonas aeruginosa (PA) remains an important pathogen in patients with cystic fibrosis (CF) lung disease as well as non-CF bronchiectasis and chronic obstructive airways disease. Initial infections are cleared but chronic infection with mucoid strains ensues in the majority of CF patients and specific interventions to prevent this critical infection transition are lacking. The PA bead model has been widely used to study pulmonary P.aeruginosa infection but has limitations in animal husbandry and in accurately mimicking human disease. We have developed an adapted agar bead murine model using a clinical mucoid strain that demonstrates the key features of transition from transitory to chronic airways infection. Infected animals show very limited acute morbidity and mortality, but undergo infection-related weight loss and neutrophilic inflammation, development of anti-pseudomonal antibodies, variable bacterial clearance, endobronchial infection and microbial adaptation with PA small colony variants. We anticipate this model will allow research into the host and microbial factors governing this critical period in Pseudomonas aeruginosa pulmonary pathogenesis when transition to chronicity is occurring.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Evans, Professor Tom and Irvine, Dr Sharon and Ritchie, Dr Neil and Bayes, Dr Hannah |
| Authors: | Bayes, H.K., Ritchie, N., Irvine, S., and Evans, T.J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Scientific Reports |
| Publisher: | Nature Research |
| ISSN: | 2045-2322 |
| ISSN (Online): | 2045-2322 |
| Copyright Holders: | Copyright © 2016 The Authors |
| First Published: | First published in Scientific Reports 6:35838 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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