In vivo derived HIV-1nef gene products are heterogeneous and lack detectable nucleotide binding activity

Harris, M., Hislop, S., Patsilinacos, P. and Neil, J. C. (1992) In vivo derived HIV-1nef gene products are heterogeneous and lack detectable nucleotide binding activity. AIDS Research and Human Retroviruses, 8(5), pp. 537-543. (doi:10.1089/aid.1992.8.537) (PMID:1515209)

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Abstract

Multiple HIV-1 nef genes were cloned from lymphocyte DNA of asymptomatic seropositive individuals by polymerase chain reaction (PCR). Sequence analysis of these clones revealed a unique set of nef variants with premature terminations (PCRnef 1 and 6), mutations at sites of potential posttranslational modification (PCRnef 2 and 3) and deletions. In common with laboratory isolates of nef, strong sequence conservation was observed in the central domain of nef and in the myristylation target sequence, with variable domains toward the N- and C-termini of the molecule. The biochemical function of nef remains elusive however, as the products of these genes cloned into a bacterial expression system failed to reveal any nucleotide binding activity.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Neil, Professor James
Authors: Harris, M., Hislop, S., Patsilinacos, P., and Neil, J. C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:AIDS Research and Human Retroviruses
Publisher:Mary Ann Liebert, Inc.
ISSN:0889-2229
ISSN (Online):1931-8405

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