Differential expression of microRNAs in bovine papillomavirus type 1 transformed equine cells

Terron-Canedo, N., Weir, W. , Nicolson, L., Britton, C. and Nasir, L. (2017) Differential expression of microRNAs in bovine papillomavirus type 1 transformed equine cells. Veterinary and Comparative Oncology, 15(3), pp. 764-774. (doi: 10.1111/vco.12216) (PMID:27039895)

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Bovine papillomavirus (BPV) types 1 and 2 play an important role in the pathogenesis of equine sarcoids (ES), the most common cutaneous tumour affecting horses. MicroRNAs (miRNAs), small non-coding RNAs that regulate essential biological and cellular processes, have been found dysregulated in a wide range of tumours. The aim of this study was to identify miRNAs associated with ES. Differential expression of miRNAs was assessed in control equine fibroblasts (EqPalFs) and EqPalFs transformed with the BPV-1 genome (S6-2 cells). Using a commercially available miRNA microarray, 492 mature miRNAs were interrogated. In total, 206 mature miRNAs were differentially expressed in EqPalFs compared with S6-2 cells. Aberrant expression of these miRNAs in S6-2 cells can be attributed to the presence of BPV-1 genomes. Furthermore, we confirm the presence of 124 miRNAs previously computationally predicted in the horse. Our data supports the involvement of miRNAs in the pathogenesis of ES.

Item Type:Articles
Additional Information:This work was kindly funded by the Horserace Betting Levy Board and the James Herriot Scholarship Fund.
Glasgow Author(s) Enlighten ID:Nasir, Professor Lubna and Britton, Professor Collette and Nicolson, Dr Lesley and Weir, Professor Willie
Authors: Terron-Canedo, N., Weir, W., Nicolson, L., Britton, C., and Nasir, L.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Veterinary and Comparative Oncology
ISSN (Online):1476-5829
Published Online:04 April 2016
Copyright Holders:Copyright © 2016 John Wiley and Sons, Ltd
First Published:First published in Veterinary and Comparative Oncology 15(3):764-774
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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