De Lamarter, J. F., Neil, J. C. , Ghysdael, J. and Vogt, P. K. (1981) The 28 S genomic RNA of avian sarcoma virus PRCII codes for the transformation-specific polyprotein P105. Virology, 112(2), pp. 757-761. (doi: 10.1016/0042-6822(81)90321-4)
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Abstract
We have characterized the genomic RNA of the defective avian sarcoma virus PRCII. Replicating virus which consists of transforming PRCII and a nontransforming but replication-competent helper, PRCII-AV, contains two RNA species. One is identical in size to the 35 S genome of avian leukosis helper viruses. The second component migrates slightly faster than 28 S ribosomal RNA in polyacrylamide gel electrophoresis but co-sediments with this RNA in sucrose gradients. In vitro translation across a gradient of velocity-sedimented poly(A)-containing PRCII virion RNA yielded three major proteins. The virion protein precursors Pr76gag and Pr180gag-pol were translated from 35 S RNA, while the transformation-specific polyprotein P105 was translated from 28 S RNA. P105 may be the only protein coded for by the PRCII genome, although this product would not exhaust the coding capacity of 28 S RNA. Whether translated in vitro or immunoprecipitated from transformed cells, P105 was essentially identical as demonstrated by comparative peptide maps.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Neil, Professor James |
Authors: | De Lamarter, J. F., Neil, J. C., Ghysdael, J., and Vogt, P. K. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Virology |
Publisher: | Elsevier |
ISSN: | 0042-6822 |
ISSN (Online): | 1096-0341 |
Published Online: | 09 June 2004 |
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