Multifaceted studies of the DNA interactions and In vitro cytotoxicity of anticancer polyaromatic platinum(II) complexes

Pages, B. J., Sakoff, J., Gilbert, J., Rodger, A., Chmel, N. P., Jones, N. C., Kelly, S. M. , Ang, D. L. and Aldrich-Wright, J. R. (2016) Multifaceted studies of the DNA interactions and In vitro cytotoxicity of anticancer polyaromatic platinum(II) complexes. Chemistry: A European Journal, 22(26), pp. 8943-8954. (doi:10.1002/chem.201601221) (PMID:27219069)

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Abstract

This study reports a detailed biophysical analysis of the DNA binding and cytotoxicity of six platinum complexes (PCs). They are of the type [Pt(PL)(SS-dach)]Cl2, where PL is a polyaromatic ligand and SS-dach is 1S,2S-diaminocyclohexane. The DNA binding of these complexes was investigated using six techniques including ultraviolet and fluorescence spectroscopy, linear dichroism, synchrotron radiation circular dichroism, isothermal titration calorimetry and mass spectrometry. This portfolio of techniques has not been extensively used to study the interactions of such complexes previously; each assay provided unique insight. The in vitro cytotoxicity of these compounds was studied in ten cell lines and compared to the effects of their R,R enantiomers; activity was very high in Du145 and SJ-G2 cells, with some submicromolar IC50 values. In terms of both DNA affinity and cytotoxicity, complexes of 5,6-dimethyl-1,10-phenanthroline and 2,2′-bipyridine exhibited the greatest and least activity, respectively, suggesting that there is some correlation between DNA binding and cytotoxicity.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kelly, Dr Sharon
Authors: Pages, B. J., Sakoff, J., Gilbert, J., Rodger, A., Chmel, N. P., Jones, N. C., Kelly, S. M., Ang, D. L., and Aldrich-Wright, J. R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Chemistry: A European Journal
Publisher:Wiley
ISSN:0947-6539
ISSN (Online):1521-3765
Published Online:24 May 2016

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