Kotze, H. L., Armitage, E. G. , Fletcher, J. S., Henderson, A., Williams, K. J., Lockyer, N. P. and Vickerman, J. C. (2012) ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems. Surface and Interface Analysis, 45(1), pp. 277-281. (doi: 10.1002/sia.5055)
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Abstract
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Armitage, Dr Emily Grace |
| Authors: | Kotze, H. L., Armitage, E. G., Fletcher, J. S., Henderson, A., Williams, K. J., Lockyer, N. P., and Vickerman, J. C. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Surface and Interface Analysis |
| Publisher: | Wiley |
| ISSN: | 0142-2421 |
| ISSN (Online): | 1096-9918 |
| Published Online: | 20 June 2012 |
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