Armitage, E. G. , Kotze, H. L., Fletcher, J. S., Henderson, A., Williams, K. J., Lockyer, N. P. and Vickerman, J. C.. (2012) Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancer. Surface and Interface Analysis, 45(1), pp. 282-285. (doi: 10.1002/sia.5050)
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Abstract
It is known that hypoxia-inducible factor 1 (HIF-1) activity results in the coordinated up-regulation of a large number of proteins that facilitate cell survival in tumours; however, the effect of HIF-1 on cancer metabolism is less well characterised. With knowledge of the specific effect of HIF-1 on cancer metabolism, biomarkers could be identified for which new drugs could be targeted. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) offers the potential to analyse intact cells in situ and has a mass spectral coverage that is applicable to metabolic profiling. It has been used to analyse the effects of HIF-1 on multicellular tumour models. Multicellular tumour spheroids (MTSs) have been cultured from human colon carcinoma cells with and without the expression of HIF-1, and the surface of the cross sections of each MTS has been analysed. Because metabolic profiling is an emerging field in ToF-SIMS, there is a requirement to determine which metabolites can be detected using this technique and which of those can be identified in complex mixtures within biological samples. For this, a selection of metabolites have been analysed, and the ToF-SIMS standard spectra acquired have been used to localise metabolites in MTS sections. The comparison of metabolic profiles of MTSs with and without the expression of HIF-1 has elucidated potential biomarkers for tumour survival in hypoxia, some of which may be HIF-1 regulated.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Armitage, Dr Emily Grace |
Authors: | Armitage, E. G., Kotze, H. L., Fletcher, J. S., Henderson, A., Williams, K. J., Lockyer, N. P., and Vickerman, J. C.. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Surface and Interface Analysis |
Publisher: | Wiley |
ISSN: | 0142-2421 |
ISSN (Online): | 1096-9918 |
Published Online: | 05 June 2012 |
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