Bursicon-α subunit modulates dLGR2 activity in the adultDrosophila melanogastermidgut independently to Bursicon-β

Scopelliti, A., Bauer, C. , Cordero, J. B. and Vidal, M. (2016) Bursicon-α subunit modulates dLGR2 activity in the adultDrosophila melanogastermidgut independently to Bursicon-β. Cell Cycle, 15(12), pp. 1538-1544. (doi: 10.1080/15384101.2015.1121334) (PMID:27191973) (PMCID:PMC4934083)

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Bursicon is the main regulator of post molting and post eclosion processes during arthropod development. The active Bursicon hormone is a heterodimer of Burs-α and Burs-β. However, adult midguts express Burs-α to regulate the intestinal stem cell niche. Here, we examined the potential expression and function of its heterodimeric partner, Burs-β in the adult midgut. Unexpectedly, our evidence suggests that Burs-β is not significantly expressed in the adult midgut. burs-β mutants displayed the characteristic developmental defects but showed wild type-like adult midguts, thus uncoupling the developmental and adult phenotypes seen in burs-α mutants. Gain of function data and ex vivo experiments using a cAMP biosensor, demonstrated that Burs-α is sufficient to drive stem cell quiescence and to activate dLGR2 in the adult midgut. Our evidence suggests that the post developmental transactivation of dLGR2 in the adult midgut is mediated by Burs-α and that the β subunit of Bursicon is dispensable for these activities.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Vidal, Dr Marcos and Cordero, Professor Julia and Bauer, Christin and Scopelliti, Mr Alessandro
Authors: Scopelliti, A., Bauer, C., Cordero, J. B., and Vidal, M.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Cell Cycle
Publisher:Taylor & Francis
ISSN (Online):1551-4005
Copyright Holders:Copyright © 2016 Alessandro Scopelliti, Christin Bauer, Julia B. Cordero, and Marcos Vidal
First Published:First published in Cell Cycle 15(12): 1538-1544
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
633021Regulation of stem cell function during tissue homeostasis and transformationJulia CorderoWellcome Trust (WELLCOME)104103/Z/14/ZRI CANCER SCIENCES