Deplanche, M. et al. (2016) Staphylococcus aureus phenol-soluble modulins impair interleukin expression in bovine mammary epithelial cells. Infection and Immunity, 84(6), pp. 1682-1692. (doi: 10.1128/iai.01330-15) (PMID:27001539)
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Abstract
The role of the recently described interleukin-32 (IL-32) in Staphylococcus aureus-induced mastitis, an inflammation of the mammary gland, is unclear. We determined expression of IL-32, IL-6, and IL-8 in S. aureus- and Escherichia coli-infected bovine mammary gland epithelial cells. Using live bacteria, we found that in S. aureus-infected cells, induction of IL-6 and IL-8 expression was less pronounced than in E. coli-infected cells. Notably, IL-32 expression was decreased in S. aureus-infected cells, while it was increased in E. coli-infected cells. We identified the staphylococcal phenol-soluble modulin (PSM) peptides as key contributors to these effects, as IL-32, IL-6, and IL-8 expression by epithelial cells exposed to psm mutant strains was significantly increased compared to that in cells exposed to the isogenic S. aureus wild-type strain, indicating that PSMs inhibit the production of these interleukins. The use of genetically complemented strains confirmed this observation. Inasmuch as the decreased expression of IL-32, which is involved in dendritic cell maturation, impairs immune responses, our results support a PSM-dependent mechanism that allows for the development of chronic S. aureus-related mastitis.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Smith, Professor David |
| Authors: | Deplanche, M., Alekseeva, L., Semenovskaya, K., Fu, C.-L., Dessauge, F., Finot, L., Petzl, W., Zerbe, H., Le Loir, Y., Rainard, P., Smith, D. G.E., Germon, P., Otto, M., Berkova, N., and Camilli, A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Infection and Immunity |
| Publisher: | American Society for Microbiology |
| ISSN: | 0019-9567 |
| ISSN (Online): | 1098-5522 |
| Published Online: | 21 March 2016 |
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