Spatially Controlled Sonoporation of Prostate Cancer Cells Via Ultrasound Activated Microbubble Cavitation

Prentice, P.A. , McLean, D., Cuschieri, A., Dholakia, K. and Campbell, P.A. (2005) Spatially Controlled Sonoporation of Prostate Cancer Cells Via Ultrasound Activated Microbubble Cavitation. In: 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology, Kahuku, HI, USA, 12-15 May 2005, pp. 158-159. ISBN 0780387112 (doi: 10.1109/MMB.2005.1548412)

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Cells that are exposed to ultrasonic (US) energy, in the presence of ultrasound contrast agent microbubbles, may experience enhanced membrane permeability. If the effective dose of US exceeds some threshold, then cell lysis can result (lethal sonoporation), however for lower doses a transient enhancement of membrane permeability occurs (reversible or non lethal sonoporation). The merits of each mode are clear: lethal sonoporation constitutes a significant tumour therapy weapon, whilst its less intrusive counterpart, reversible sonoporation, represents an effective non-invasive targeted drug delivery technique. Until now, the mechanism of the dynamic interaction between microbubbles and cells has remained unknown. Moreover pores, which are the presumed mode of permeabilization have not been observed in a convincing fashion. We will demonstrate, for the first time, how an innovative hybridization of holographic optical trapping technology, together with the application of MHz pulsed US energy and subsequent high resolution observation using atomic force microscopy has been used to elucidate the fundamental mode for membrane permeabilization during sonoporation.

Item Type:Conference Proceedings
Glasgow Author(s) Enlighten ID:Prentice, Dr Paul
Authors: Prentice, P.A., McLean, D., Cuschieri, A., Dholakia, K., and Campbell, P.A.
College/School:College of Science and Engineering > School of Engineering > Systems Power and Energy

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