Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects

Child, H. W., Hernandez, Y., Conde, J., Mullin, M., Baptista, P., de la Fuente, J. M. and Berry, C. (2015) Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects. Nanomedicine, 10(16), pp. 2513-2525. (doi: 10.2217/NNM.15.95) (PMID:26302331)

Abstract

Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Berry, Dr Catherine and Mullin, Mrs Margaret and Child, Dr Hannah
• Authors: Child, H. W., Hernandez, Y., Conde, J., Mullin, M., Baptista, P., de la Fuente, J. M., and Berry, C.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; College of Medical Veterinary and Life Sciences; College of Medical Veterinary and Life Sciences > School of Life Sciences
• Journal Name: Nanomedicine
• Publisher: Future Medicine
• ISSN: 1743-5889
• ISSN (Online): 1748-6963