Roberts, J. N. et al. (2016) Dynamic surfaces for the study of mesenchymal stem cell growth through adhesion regulation. ACS Nano, 10(7), pp. 6667-6679. (doi: 10.1021/acsnano.6b01765) (PMID:27322014) (PMCID:PMC4963921)
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Abstract
Out of their niche environment, adult stem cells, such as mesenchymal stem cells (MSCs), spontaneously differentiate. This makes both studying these important regenerative cells and growing large numbers of stem cells for clinical use challenging. Traditional cell culture techniques have fallen short of meeting this challenge, but materials science offers hope. In this study we have used emerging rules of managing adhesion/cytoskeletal balance to prolong MSC cultures by fabricating controllable nanoscale cell interfaces using immobilized peptides that may be enzymatically activated to change their function. The surfaces can be altered (activated) at will to tip adhesion/cytoskeletal balance and initiate differentiation hence better informing biological mechanisms of stem cell growth. Tools that are able to investigate the stem cell phenotype are important. While large phenotypical differences, such as the difference between an adipocyte and an osteoblast, are now better understood, the far more subtle differences between e.g. fibroblasts and MSCs are much harder to dissect. The development of technologies able to dynamically navigate small differences in adhesion are critical in the race to provide regenerative strategies using stem cells.
| Item Type: | Articles |
|---|---|
| Additional Information: | Also funded by BB/G010579/1 |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Hay, Mr Jake and Yarwood, Dr Stephen and Roberts, Dr Jemma and Dalby, Professor Matthew and Anderson, Ms Hilary and Alakpa, Miss Enateri and Burgess, Dr Karl and McNamara, Dr Laura and Turner, Dr Lesley-Anne and Yang, Dr Jingli |
| Authors: | Roberts, J. N., Sahoo, J. K., McNamara, L. E., Burgess, K. V., Yang, J., Alakpa, E. V., Anderson, H. J., Hay, J., Turner, L.-A., Yarwood, S. J., Zelzer, M., Oreffo, R. O.C., Ulijn, R. V., and Dalby, M. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | ACS Nano |
| Publisher: | American Chemical Society |
| ISSN: | 1936-0851 |
| ISSN (Online): | 1936-086X |
| Published Online: | 20 June 2016 |
| Copyright Holders: | Copyright © 2016 The Authors |
| First Published: | First published in ACS Nano 10(7):66667-6679 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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